PyGUS: Quantitative analysis of GUS expression levels in plants, as well as plant area and expression area.

This software can accurately extract the main body of the plant from the GUS staining image, eliminating interference from opaque areas and background impurities, accurately identifying positive expression areas. At the same time, it quantitatively detects the intensity of GUS signals in the expression areas, and finally outputs the intensity of GUS signals, positive expression area, overall plant area and expression ratio.

Install

See user manual (link1, link2) or the demo video below. NOTE:1. Images names cannot contain blank space and Chinese. 2. In Mode 4, after selecting each regions, please press any button in KEYBOARD to continue .

If you want to perform statistical analysis of samples between groups, please refer to this instruction.